Chromosomal integration vectors allowing flexible expression of foreign genes in Campylobacter jejuni.

ABSTRACT:

Background

Campylobacter jejuni is a major cause of human gastroenteritis yet there is limited knowledge of how disease is caused. Molecular genetic approaches are vital for research into the virulence mechanisms of this important pathogen. Vectors that allow expression of genes in C. jejuni via recombination onto the chromosome are particularly useful for genetic complementation of insertional knockout mutants and more generally for expression of genes in particular C. jejuni host backgrounds.

Methods

A series of three vectors that allow integration of genes onto the C. jejuni chromosome were constructed by standard cloning techniques with expression driven from three different strong promoters. Following integration onto the C. jejuni chromosome expression levels were quan

SUBMITTER: Jervis AJ 

PROVIDER: S-EPMC4619491 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature