TrkA is amplified in malignant melanoma patients and induces an anti-proliferative response in cell lines.

ABSTRACT:

Background

The nerve growth factor (NGF) receptor tyrosine-kinase TrkA is a well-known determinant of the melanocytic lineage, through modulation of the MAPK and AKT cascades. While TrkA gene is frequently rearranged in cancers, its involvement in malignant melanoma (MM) development is still unclear.

Methods

We analyzed a dataset of primary cutaneous MM (n = 31) by array comparative genomic hybridization (aCGH), to identify genomic amplifications associated with tumor progression. The analysis was validated by genomic quantitative PCR (qPCR) on an extended set of cases (n = 64) and the results were correlated with the clinical outcome. To investigate TrkA molecular pathways and cellular function, we generated inducible activation of the NGF-TrkA signaling in human MM cell li

SUBMITTER: Pasini L 

PROVIDER: S-EPMC4619539 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature