Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein.

ABSTRACT: Dengue virus (DENV) is a mosquito-borne flavivirus that causes serious human disease and mortality worldwide. There is no specific antiviral therapy or vaccine for DENV infection. Alterations in gene expression during DENV infection of the mosquito and the impact of these changes on virus infection are important events to investigate in hopes of creating new treatments and vaccines. We previously identified 203 genes that were ≥5-fold differentially upregulated during flavivirus infection of the mosquito. Here, we examined the impact of silencing 100 of the most highly upregulated gene targets on DENV infection in its mosquito vector. We identified 20 genes that reduced DENV infection by at least 60% when silenced. We focused on one gene, a putative cysteine rich venom protein (SeqID AAEL0

SUBMITTER: Londono-Renteria B 

PROVIDER: S-EPMC4619585 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature