Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae.

ABSTRACT: Nucleosome organization exhibits dynamic properties depending on the cell state and environment. Histone proteins, fundamental components of nucleosomes, are subject to chemical modifications on particular residues. We examined the effect of substituting modifiable residues of four core histones with the non-modifiable residue alanine on nucleosome dynamics. We mapped the genome-wide nucleosomes in 22 histone mutants of Saccharomyces cerevisiae and compared the nucleosome alterations relative to the wild-type strain. Our results indicated that different types of histone mutation resulted in different phenotypes and a distinct reorganization of nucleosomes. Nucleosome occupancy was altered at telomeres, but not at centromeres. The first nucleosomes upstream (-1) and downstream (+1) of the t

SUBMITTER: Liu H 

PROVIDER: S-EPMC4620441 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature