Spatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague.

ABSTRACT:

Unlabelled

During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence are poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture microdissection and transcriptome sequencing (RNA-seq) analysis to identify transcriptional differences between lesion microenvironments. We show that cellular pathways involved in leukocyte migration and apoptosis are downregulated in the center of lung lesions compared to the periphery. Probing for the bacterial factor(s) important for the alteration in neutrophil survival, we show both in vitro and in vivo that Y. pestis increases neutrophil survival in a manner that is depend

SUBMITTER: Stasulli NM 

PROVIDER: S-EPMC4620470 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature