Improvement of Cell Survival During Human Pluripotent Stem Cell Definitive Endoderm Differentiation.

ABSTRACT: Definitive endoderm (DE) is a vital precursor for internal organs such as liver and pancreas. Efficient protocol to differentiate human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) to DE is essential for regenerative medicine and for modeling diseases; yet, poor cell survival during DE differentiation remains unsolved. In this study, our use of B27 supplement in modified differentiation protocols has led to a substantial improvement. We used an SOX17-enhanced green fluorescent protein (eGFP) reporter hESC line to compare and modify established DE differentiation protocols. Both total live cell numbers and the percentages of eGFP-positive cells were used to assess differentiation efficiency. Among tested protocols, three modified protocols with serum-free B27 suppl

SUBMITTER: Wang H 

PROVIDER: S-EPMC4620527 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature