SOX15 governs transcription in human stratified epithelia and a subset of esophageal adenocarcinomas.

ABSTRACT:

Background & aims

Intestinal metaplasia (Barrett's esophagus, BE) is the principal risk factor for esophageal adenocarcinoma (EAC). Study of the basis for BE has centered on intestinal factors, but loss of esophageal identity likely also reflects absence of key squamous-cell factors. As few determinants of stratified epithelial cell-specific gene expression are characterized, it is important to identify the necessary transcription factors.

Methods

We tested regional expression of mRNAs for all putative DNA-binding proteins in the mouse digestive tract and verified esophagus-specific factors in human tissues and cell lines. Integration of diverse data defined a human squamous esophagus-specific transcriptome. We used chromatin immunoprecipitation (ChIP-seq) to locate transcri

SUBMITTER: Sulahian R 

PROVIDER: S-EPMC4620585 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature