The molecular characterisation of Escherichia coli K1 isolated from neonatal nasogastric feeding tubes.

ABSTRACT:

Background

The most common cause of Gram-negative bacterial neonatal meningitis is E. coli K1. It has a mortality rate of 10-15 %, and neurological sequelae in 30-50 % of cases. Infections can be attributable to nosocomial sources, however the pre-colonisation of enteral feeding tubes has not been considered as a specific risk factor.

Methods

Thirty E. coli strains, which had been isolated in an earlier study, from the residual lumen liquid and biofilms of neonatal nasogastric feeding tubes were genotyped using pulsed-field gel electrophoresis, and 7-loci multilocus sequence typing. Potential pathogenicity and biofilm associated traits were determined using specific PCR probes, genome analysis, and in vitro tissue culture assays.

Results

The E. coli strains clustered

SUBMITTER: Alkeskas A 

PROVIDER: S-EPMC4620641 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature