MicroRNA-143 Activation Regulates Smooth Muscle and Endothelial Cell Crosstalk in Pulmonary Arterial Hypertension.

ABSTRACT:

Rationale

The pathogenesis of pulmonary arterial hypertension (PAH) remains unclear. The 4 microRNAs representing the miR-143 and miR-145 stem loops are genomically clustered.

Objective

To elucidate the transcriptional regulation of the miR-143/145 cluster and the role of miR-143 in PAH.

Methods and results

We identified the promoter region that regulates miR-143/145 microRNA expression in pulmonary artery smooth muscle cells (PASMCs). We mapped PAH-related signaling pathways, including estrogen receptor, liver X factor/retinoic X receptor, transforming growth factor-β (Smads), and hypoxia (hypoxia response element), that regulated levels of all pri-miR stem loop transcription and resulting microRNA expression. We observed that miR-143-3p is selectively upregulated co

SUBMITTER: Deng L 

PROVIDER: S-EPMC4620852 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature