Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization.

ABSTRACT: The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during Ras(V12) immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validate

SUBMITTER: Dequeant ML 

PROVIDER: S-EPMC4620889 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature