Early aggregation preceding the nucleation of insulin amyloid fibrils as monitored by small angle X-ray scattering.

ABSTRACT: The nucleation event of amyloid fibrils is one of the most crucial processes that dictate the timing and rate of the pathology of diseases; however, information regarding how protein molecules associate to produce fibril nuclei is currently limited. In order to explore this issue in more detail, we performed time-resolved small angle X-ray scattering (SAXS) measurements on insulin fibrillation, in combination with additional multidirectional analyses of thioflavin T fluorescence, FTIR spectroscopy, light scattering, and light transmittance, during the fibrillation process of bovine insulin. SAXS monitoring revealed that insulin molecules associated into rod-like prefibrillar aggregates in the very early stage of the reaction. After the formation of these early aggregates, they appeared to

SUBMITTER: Chatani E 

PROVIDER: S-EPMC4621412 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature