Studies on microsatellite instability in p16 gene and expression of hMSH2 mRNA in human gastric cancer tissues.

ABSTRACT: AIM:To detect the loss of heterozygosity (LOH) frequency of microsatellite sites D9s171, D9s1604 of p16 gene and expression of hMSH2 mRNA in various differentiated types of gastric cancer, adjacent cancer tissues and normal gastric mucosa. METHODS:LOH was detected by polymerase chain reaction (PCR)-denaturing polyacrylamide gel electrophoresis-silver staining. The expression of hMSH2 mRNA was examined with in situ hybridization. RESULTS:The frequency rate of LOH was significantly higher in gastric cancers than that in adjacent cancer tissues (P=0.032). No significant difference was noted among various differentiated types and various clinical stages of gastric cancers. The significantly reduced expression of hMSH2 mRNA positive signal cells exhibited in gastric cancers, in comparison with

SUBMITTER: Zhang QX 

PROVIDER: S-EPMC4621556 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature