SATB1 Promotes Pancreatic Cancer Growth and Invasion Depending on MYC Activation.

ABSTRACT:

Background

SATB1 plays an important role in human malignant progression, inducing cancer cell proliferation and metastasis by regulating downstream gene expressions. However, little is known about the underlying mechanisms in which SATB1 promotes pancreatic cancer tumorigenesis.

Aims

To investigate SATB1 expression levels and its biological functions in promoting pancreatic cancer growth and invasion.

Methods

SATB1 expression levels were detected in seven human pancreatic cancer cell lines and 16 pairs of normal pancreatic/pancreatic cancer tissues using RT-PCR and western blot. SW1990 or Capan-1 cells stably knockdown (shRNA) or transiently knockdown (siRNA) SATB1 cells, and PANC-1 stably overexpressing SATB1 cells were investigated with MTT, EdU assay, flow cytometr

SUBMITTER: Chen Z 

PROVIDER: S-EPMC4621700 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature