Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines.

ABSTRACT: Bioluminescent imaging is an emerging biomedical surveillance strategy that uses external cameras to detect in vivo light generated in small animal models of human physiology or in vitro light generated in tissue culture or tissue scaffold mimics of human anatomy. The most widely utilized of reporters is the firefly luciferase (luc) gene; however, it generates light only upon addition of a chemical substrate, thus only generating intermittent single time point data snapshots. To overcome this disadvantage, we have demonstrated substrate-independent bioluminescent imaging using an optimized bacterial bioluminescence (lux) system. The lux reporter produces bioluminescence autonomously using components found naturally within the cell, thereby allowing imagin

SUBMITTER: Xu T 

PROVIDER: S-EPMC4621765 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature