Characterizing monoclonal antibody structure by carbodiimide/GEE footprinting.

ABSTRACT: Amino acid-specific covalent labeling is well suited to probe protein structure and macromolecular interactions, especially for macromolecules and their complexes that are difficult to examine by alternative means, due to size, complexity, or instability. Here we present a detailed account of carbodiimide-based covalent labeling (with GEE tagging) applied to a glycosylated monoclonal antibody therapeutic, which represents an important class of biologic drugs. Characterization of such proteins and their antigen complexes is essential to development of new biologic-based medicines. In this study, the experiments were optimized to preserve the structural integrity of the protein, and experimental conditions were varied and replicated to establish the reproducibility and precision of the techn

SUBMITTER: Kaur P 

PROVIDER: S-EPMC4622050 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature