Potential therapeutic strategy for gastric cancer peritoneal metastasis by NKG2D ligands-specific T cells.

ABSTRACT:

Purpose

Despite advancements in its treatment, gastric cancer continues to be one of the leading causes of cancer deaths worldwide. Adoptive transfer of chimeric antigen receptor-modified T cells is a promising antitumor therapy for many cancers. The purpose of this study was to construct a chimeric receptor linking the extracellular domain of NKG2D to the CD28 and CD3zeta chain intracellular domains to target gastric cancers that expressed NKG2D ligands.

Methods

Expression of NKG2D ligands including MICA, MICB, and ULBP1-3 in a gastric cancer cell line and primary gastric cancer cells from ascites samples were analyzed using flow cytometry. Co-culture experiments were performed by incubating chNKG2D T cells with gastric cancer cell lines and with primary human gastric cance

SUBMITTER: Liu X 

PROVIDER: S-EPMC4622417 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature