MiR-33a functions as a tumor suppressor in melanoma by targeting HIF-1α.

ABSTRACT:

Background

Our previous findings showed that miR-33 expressed abnormally in clinical specimens of melanoma, but the exact molecular mechanism has not been elucidated.

Object

To determine miR-33's roles in melanoma and confirm whether HIF-1α is a direct target gene of miR-33a.

Methods

First miR-33a/b expression levels were detected in HM, WM35, WM451, A375 and SK-MEL-1. Then lentiviral vectors were constructed to intervene miR-33a expression in melanoma cells. Cell proliferation, invasion and metastasis were detected. A375 cells mice model was performed to test the tumorigenesis of melanoma in vivo. Finally the dual reporter gene assay was carried out to confirm whether HIF-1α is a direct target gene of miR-33a.

Results

MiR-33a/b exhibited a lower expression in

SUBMITTER: Zhou J 

PROVIDER: S-EPMC4622471 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature