High throughput functional epitope mapping: revisiting phage display platform to scan target antigen surface.

ABSTRACT: Antibody engineering must be accompanied by mapping strategies focused on identifying the epitope recognized by each antibody to define its unique functional identity. High throughput fine specificity determination remains technically challenging. We review recent experiences aimed at revisiting the oldest and most extended display technology to develop a robust epitope mapping platform, based on the ability to manipulate target-derived molecules (ranging from the whole native antigen to antigen domains and smaller fragments) on filamentous phages. Single, multiple and combinatorial mutagenesis allowed comprehensive scanning of phage-displayed antigen surface that resulted in the identification of clusters of residues contributing to epitope formation. Functional pictures of the epitope(s)

SUBMITTER: Rojas G 

PROVIDER: S-EPMC4622681 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature