Tea pigments induce cell-cycle arrest and apoptosis in HepG2 cells.

ABSTRACT:

Aim

To investigate the molecular mechanisms by which tea pigments exert preventive effects on liver carcinogenesis.

Methods

HepG2 cells were seeded at a density of 5X10(5)/well in six-well culture dishes and incubated overnight. The cells then were treated with various concentrations of tea pigments over 3 d, harvested by trypsinization, and counted using a hemocytometer. Flow cytometric analysis was performed by a flow cytometer after propidium iodide labeling. Bcl-2 and p21(WAF1) proteins were determined by Western blotting. In addition, DNA laddering assay was performed on treated and untreated cultured HepG2 cells.

Results

Tea pigments inhibited the growth of HepG2 cells in a dose-dependent manner. Flow-cytometric analysis showed that tea pigments arrested cell cy

SUBMITTER: Jia XD 

PROVIDER: S-EPMC4622794 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature