Using RNA sequencing for identifying gene imprinting and random monoallelic expression in human placenta.

ABSTRACT: Given the possible critical importance of placental gene imprinting and random monoallelic expression on fetal and infant health, most of those genes must be identified, in order to understand the risks that the baby might meet during pregnancy and after birth. Therefore, the aim of the current study was to introduce a workflow and tools for analyzing imprinted and random monoallelic gene expression in human placenta, by applying whole-transcriptome (WT) RNA sequencing of placental tissue and genotyping of coding DNA variants in family trios. Ten family trios, each with a healthy spontaneous single-term pregnancy, were recruited. Total RNA was extracted for WT analysis, providing the full sequence information for the placental transcriptome. Parental and child blood DNA genotypes were anal

SUBMITTER: Metsalu T 

PROVIDER: S-EPMC4623103 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature