DNA microarray revealed and RNAi plants confirmed key genes conferring low Cd accumulation in barley grains.

ABSTRACT:

Background

Understanding the mechanism of low Cd accumulation in crops is crucial for sustainable safe food production in Cd-contaminated soils.

Results

Confocal microscopy, atomic absorption spectrometry, gas exchange and chlorophyll fluorescence analyses revealed a distinct difference in Cd accumulation and tolerance between the two contrasting barley genotypes: W6nk2 (a low-grain-Cd-accumulating and Cd-sensitive genotype) and Zhenong8 (a high-grain-Cd-accumulating and tolerant genotype). A DNA microarray analysis detected large-scale changes of gene expression in response to Cd stress with a substantial difference between the two genotypes. Cd stress led to higher expression of genes involved in transport, carbohydrate metabolism and signal transduction in the low-grain-C

SUBMITTER: Sun H 

PROVIDER: S-EPMC4623906 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature