Characterizing Cellular Biophysical Responses to Stress by Relating Density, Deformability, and Size.

ABSTRACT: Cellular physical properties are important indicators of specific cell states. Although changes in individual biophysical parameters, such as cell size, density, and deformability, during cellular processes have been investigated in great detail, relatively little is known about how they are related. Here, we use a suspended microchannel resonator (SMR) to measure single-cell density, volume, and passage time through a narrow constriction of populations of cells subjected to a variety of environmental stresses. Osmotic stress significantly affects density and volume, as previously shown. In contrast to density and volume, the effect of an osmotic challenge on passage time is relatively small. Deformability, as determined by comparing passage times for cells with similar volume, exhibits a

SUBMITTER: Byun S 

PROVIDER: S-EPMC4624161 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature