Quantification of ¹H NMR Spectra from Human Plasma.

ABSTRACT: Human plasma is a biofluid that is high in information content, making it an excellent candidate for metabolomic studies. ¹H NMR has been a popular technique to detect several dozen metabolites in blood plasma. In order for ¹H NMR to become an automated, high-throughput method, challenges related to (1) the large signal from lipoproteins and (2) spectral overlap between different metabolites have to be addressed. Here diffusion-weighted ¹H NMR is used to separate lipoprotein and metabolite signals based on their large difference in translational diffusion. The metabolite ¹H NMR spectrum is then quantified through spectral fitting utilizing full prior knowledge on the metabolite spectral signatures. Extension of the scan time by 3 minutes or 15% p

SUBMITTER: de Graaf RA 

PROVIDER: S-EPMC4624446 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature