Expression of a Chimeric Antigen Receptor in Multiple Leukocyte Lineages in Transgenic Mice.

ABSTRACT: Genetically modified CD8+ T lymphocytes have shown significant anti-tumor effects in the adoptive immunotherapy of cancer, with recent studies highlighting a potential role for a combination of other immune subsets to enhance these results. However, limitations in present genetic modification techniques impose difficulties in our ability to fully explore the potential of various T cell subsets and assess the potential of other leukocytes armed with chimeric antigen receptors (CARs). To address this issue, we generated a transgenic mouse model using a pan-hematopoietic promoter (vav) to drive the expression of a CAR specific for a tumor antigen. Here we present a characterization of the immune cell compartment in two unique vav-CAR transgenic mice models, Founder 9 (F9) and Founder 38 (F38)

SUBMITTER: Yong CS 

PROVIDER: S-EPMC4624721 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature