A high-throughput RNA-seq approach to profile transcriptional responses.

ABSTRACT: In recent years RNA-seq protocols have been developed to investigate a variety of biological problems by measuring the abundance of different RNAs. Many study designs involve performing expensive preliminary studies to screen or optimize experimental conditions. Testing a large number of conditions in parallel may be more cost effective. For example, analyzing tissue/environment-specific gene expression generally implies screening a large number of cellular conditions and samples, without prior knowledge of which conditions are most informative (e.g., some cell types may not respond to certain treatments). To circumvent these challenges, we have established a new two-step high-throughput RNA-seq approach: the first step consists of gene expression screening of a large number of conditions,

SUBMITTER: Moyerbrailean GA 

PROVIDER: S-EPMC4625130 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature