Integration of Ixodes ricinus genome sequencing with transcriptome and proteome annotation of the naive midgut.

ABSTRACT:

Background

In Europe, Ixodes ricinus ticks are the most important vectors of diseases threatening humans, livestock, wildlife and companion animals. Nevertheless, genomic sequence information is missing and functional annotation of transcripts and proteins is limited. This lack of information is restricting studies of the vector and its interactions with pathogens and hosts. Here we present and integrate the first analysis of the I. ricinus genome with the transcriptome and proteome of the unfed I. ricinus midgut.

Methods

Whole genome sequencing was performed on I. ricinus ticks and the sequences were de novo assembled. In parallel, I. ricinus ticks were dissected and the midgut transcriptome sequenced. Both datasets were integrated by transcript discovery analysis to identi

SUBMITTER: Cramaro WJ 

PROVIDER: S-EPMC4625525 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature