Development and validation of an ELISA kit (YF MAC-HD) to detect IgM to yellow fever virus.

ABSTRACT: Yellow fever virus (YFV) is endemic in tropical and sub-tropical regions of the world, with around 180,000 human infections a year occurring in Africa. Serologic testing is the chief laboratory diagnostic means of identifying an outbreak and to inform the decision to commence a vaccination campaign. The World Health Organization disseminates the reagents for YFV testing to African reference laboratories, and the US Centers for Disease Control and Prevention (CDC) is charged with producing and providing these reagents. The CDC M-antibody capture ELISA is a 2-day test, requiring titration of reagents when new lots are received, which leads to inconsistency in testing and wastage of material. Here we describe the development of a kit-based assay (YF MAC-HD) based upon the CDC method, that is

SUBMITTER: Basile AJ 

PROVIDER: S-EPMC4625539 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature