Deep Sequencing in Microdissected Renal Tubules Identifies Nephron Segment-Specific Transcriptomes.

ABSTRACT: The function of each renal tubule segment depends on the genes expressed therein. High-throughput methods used for global profiling of gene expression in unique cell types have shown low sensitivity and high false positivity, thereby limiting the usefulness of these methods in transcriptomic research. However, deep sequencing of RNA species (RNA-seq) achieves highly sensitive and quantitative transcriptomic profiling by sequencing RNAs in a massive, parallel manner. Here, we used RNA-seq coupled with classic renal tubule microdissection to comprehensively profile gene expression in each of 14 renal tubule segments from the proximal tubule through the inner medullary collecting duct of rat kidneys. Polyadenylated mRNAs were captured by oligo-dT primers and processed into adapter-ligated cDN

SUBMITTER: Lee JW 

PROVIDER: S-EPMC4625681 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature