Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood.

ABSTRACT:

Background

Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In spite of this, at a global level, pneumococcal infection remains an important cause of death among children under 5 years of age and in adults 65 years of age or older. In order to properly manage patients and control the spread of infection, a rapid and highly sensitive diagnostic method is needed for routine implementation, especially in resource-limited regions where pneumococcal disease is most prevalent.

Methods

Using the gene encoding leader peptidase A as a molecular diagnostics target, a real-time RPA assay was designed and optimised for the detection

SUBMITTER: Clancy E 

PROVIDER: S-EPMC4625855 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature