Genome-wide identification and evaluation of constitutive promoters in streptomycetes.

ABSTRACT:

Background

Streptomycetes attract a lot of attention in metabolic engineering and synthetic biology because of their well-known ability to produce secondary metabolites. However, the available constitutive promoters are rather limited in this genus.

Results

In this work, constitutive promoters were selected from a pool of promoters whose downstream genes maintained constant expression profiles in various conditions. A total of 941 qualified genes were selected based on systematic analysis of five sets of time-series transcriptome microarray data of Streptomyces coelicolor M145 cultivated under different conditions. Then, 166 putative constitutive promoters were selected by following a rational selection workflow containing disturbance analysis, function analysis, genetic loc

SUBMITTER: Li S 

PROVIDER: S-EPMC4625935 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature