Early enhancer establishment and regulatory locus complexity shape transcriptional programs in hematopoietic differentiation.

ABSTRACT: We carried out an integrative analysis of enhancer landscape and gene expression dynamics during hematopoietic differentiation using DNase-seq, histone mark ChIP-seq and RNA sequencing to model how the early establishment of enhancers and regulatory locus complexity govern gene expression changes at cell state transitions. We found that high-complexity genes-those with a large total number of DNase-mapped enhancers across the lineage-differ architecturally and functionally from low-complexity genes, achieve larger expression changes and are enriched for both cell type-specific and transition enhancers, which are established in hematopoietic stem and progenitor cells and maintained in one differentiated cell fate but lost in others. We then developed a quantitative model to accurately predi

SUBMITTER: Gonzalez AJ 

PROVIDER: S-EPMC4626279 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature