Partial denervation of sub-basal axons persists following debridement wounds to the mouse cornea.

ABSTRACT: Although sensory reinnervation occurs after injury in the peripheral nervous system, poor reinnervation in the elderly and those with diabetes often leads to pathology. Here we quantify sub-basal axon density in the central and peripheral mouse cornea over time after three different types of injury. The mouse cornea is highly innervated with a dense array of sub-basal nerves that form a spiral called the vortex at the corneal center or apex; these nerves are readily detected within flat mounted corneas. After anesthesia, corneal epithelial cells were removed using either a dulled blade or a rotating burr within an area demarcated centrally with a 1.5 mm trephine. A third wound type, superficial trephination, involved demarcating the area with the 1.5 mm trephine but not removing cells. By

SUBMITTER: Pajoohesh-Ganji A 

PROVIDER: S-EPMC4626298 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature