Microbiome composition and geochemical characteristics of deep subsurface high-pressure environment, Pyhasalmi mine Finland.

ABSTRACT: Pyhäsalmi mine in central Finland provides an excellent opportunity to study microbial and geochemical processes in a deep subsurface crystalline rock environment through near-vertical drill holes that reach to a depth of more than two kilometers below the surface. However, microbial sampling was challenging in this high-pressure environment. Nucleic acid yields obtained were extremely low when compared to the cell counts detected (1.4 × 10(4) cells mL(-1)) in water. The water for nucleic acid analysis went through high decompression (60-130 bar) during sampling, whereas water samples for detection of cell counts by microscopy could be collected with slow decompression. No clear cells could be identified in water that went through high decompression. The high-pressure decompression may hav

SUBMITTER: Miettinen H 

PROVIDER: S-EPMC4626562 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature