Fluorescently-tagged anti-ganglioside antibody selectively identifies peripheral nerve in living animals.

ABSTRACT: Selective in vivo delivery of cargo to peripheral nervous system (PNS) has broad clinical and preclinical applications. An important applicability of this approach is systemic delivery of fluorescently conjugated ligands that selectively label PNS, which could allow visualization of peripheral nerves during any surgery. We examine the use of an anti-ganglioside monoclonal antibody (mAb) as selective neuronal delivery vector for surgical imaging of peripheral nerves. Systemic delivery of an anti-ganglioside mAb was used for selective intraneuronal/axonal delivery of fluorescent agents to visualize nerves by surgical imaging in living mice. In this study, we show that intact motor, sensory, and autonomic nerve fibers/paths are distinctly labeled following a single nanomolar systemic injectio

SUBMITTER: Massaad CA 

PROVIDER: S-EPMC4626805 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature