Microarray profiling shows distinct differences between primary tumors and commonly used preclinical models in hepatocellular carcinoma.

ABSTRACT:

Background

Despite advances in therapeutics, outcomes for hepatocellular carcinoma (HCC) remain poor and there is an urgent need for efficacious systemic therapy. Unfortunately, drugs that are successful in preclinical studies often fail in the clinical setting, and we hypothesize that this is due to functional differences between primary tumors and commonly used preclinical models. In this study, we attempt to answer this question by comparing tumor morphology and gene expression profiles between primary tumors, xenografts and HCC cell lines.

Methods

Hep G2 cell lines and tumor cells from patient tumor explants were subcutaneously (ectopically) injected into the flank and orthotopically into liver parenchyma of Mus Musculus SCID mice. The mice were euthanized after two week

SUBMITTER: Wang W 

PROVIDER: S-EPMC4628260 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature