A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates.

ABSTRACT:

Background

Columnaris disease caused by Flavobacterium columnare is a serious problem in aquaculture, annually causing large economic losses around the world. Despite considerable research, the molecular epidemiology of F. columnare remains poorly understood.

Methods

We investigated the population structure and spatiotemporal changes in the genetic diversity of F. columnare population in Finland by using a multilocus sequence typing (MLST) and analysis (MLSA) based on DNA sequence variation within six housekeeping genes. A total of 83 strains of F. columnare were collected from eight different areas located across the country between 2003 and 2012.

Results

Partial sequencing of six housekeeping genes (trpB, tuf, atpA, rpoD, gyrB and dnaK) revealed eight sequence types

SUBMITTER: Ashrafi R 

PROVIDER: S-EPMC4628280 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature