MicroRNA and mRNA expression profiling analysis revealed the regulation of plant height in Gossypium hirsutum.

ABSTRACT:

Background

Dwarf cottons are more resistant to damage from wind and rain and associated with stable, increased yields, and also desirable source for breeding the machine harvest varieties. In an effort to uncover the transcripts and miRNA networks involved in plant height, the transcriptome and small RNA sequencing were performed based on dwarf mutant Ari1327 (A1), tall-culm mutant Ari3697 (A3) and wild type Ari971 (A9) in Gossypium hirsutum.

Methods

The stem apexes of wild-type upland cotton (Ari971) and its dwarf mutant (Ari1327) and tall-culm mutant (Ari3697) at the fifth true leaf stage were extracted for RNA, respectively. Transcriptome and small RNA libraries were constructed and subjected to next generation sequencing.

Results

The transcriptome sequencing analy

SUBMITTER: An W 

PROVIDER: S-EPMC4628322 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature