Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland.

ABSTRACT: The silkworm silk glands are powerful secretory organs that can produce and secrete proteins at high levels. As such, it has been suggested that the biosynthetic and secretory power of the silk gland can be harnessed to produce and secrete recombinant proteins in tight or loose association with silk fibers. However, the utility of the silkworm platform is constrained by the fact that it has a relatively primitive protein N-glycosylation pathway, which produces relatively simple insect-type, rather than mammalian-type N-glycans. In this study, we demonstrate for the first time that the silk gland protein N-glycosylation pathway can be glycoengineered. We accomplished this by using a dual piggyBac vector encoding two distinct mammalian glycosyltransferases under the transcriptional control o

SUBMITTER: Mabashi-Asazuma H 

PROVIDER: S-EPMC4628589 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature