Initial amplification of the HPV18 genome proceeds via two distinct replication mechanisms.

ABSTRACT: Determining the mechanism of HPV18 replication is paramount for identifying possible drug targets against HPV infection. We used two-dimensional and three-dimensional gel electrophoresis techniques to identify replication intermediates arising during the initial amplification of HPV18 episomal genomes. We determined that the first rounds of HPV18 replication proceed via bidirectional theta structures; however, a notable accumulation of almost fully replicated HPV18 genomes indicates difficulties with the completion of theta replication. We also observed intermediates that were created by a second replication mechanism during the initial amplification of HPV18 genomes. The second replication mechanism does not utilize specific initiation or termination sequences and proceeds via a unidirect

SUBMITTER: Orav M 

PROVIDER: S-EPMC4629122 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature