Integrated micro-optofluidic platform for real-time detection of airborne microorganisms.

ABSTRACT: We demonstrate an integrated micro-optofluidic platform for real-time, continuous detection and quantification of airborne microorganisms. Measurements of the fluorescence and light scattering from single particles in a microfluidic channel are used to determine the total particle number concentration and the microorganism number concentration in real-time. The system performance is examined by evaluating standard particle measurements with various sample flow rates and the ratios of fluorescent to non-fluorescent particles. To apply this method to real-time detection of airborne microorganisms, airborne Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis cells were introduced into the micro-optofluidic platform via bioaerosol generation, and a liquid-type particle collecti

SUBMITTER: Choi J 

PROVIDER: S-EPMC4629162 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature