Production of hyperpolarized ¹³CO₂ from [1-¹³C]pyruvate in perfused liver does reflect total anaplerosis but is not a reliable biomarker of glucose production.

ABSTRACT: In liver, ¹³CO₂ can be generated from [1-¹³C] pyruvate via pyruvate dehydrogenase or anaplerotic entry of pyruvate into the TCA cycle followed by decarboxylation at phosphoenolpyruvate carboxykinase (PEPCK), the malic enzyme, isocitrate dehydrogenase, or α-ketoglutarate dehydrogenase. The purpose of this study was to determine the relative importance of these pathways in production of hyperpolarized (HP) ¹³CO₂ after administration of hyper-polarized pyruvate in livers supplied with a fatty acid plus substrates for gluconeogenesis. Isolated mouse livers were perfused with a mixture of thermally-polarized ¹³C-enriched pyruvate, lactate and octanoate in various combinations prior to exposure to HP pyruvate. Under all perfusion

SUBMITTER: Moreno KX 

PROVIDER: S-EPMC4629494 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature