Alcohol directly stimulates epigenetic modifications in hepatic stellate cells.

ABSTRACT:

Background & aims

Alcohol is a primary cause of liver disease and an important co-morbidity factor in other causes of liver disease. A common feature of progressive liver disease is fibrosis, which results from the net deposition of fibril-forming extracellular matrix (ECM). The hepatic stellate cell (HSC) is widely considered to be the major cellular source of fibrotic ECM. We determined if HSCs are responsive to direct stimulation by alcohol.

Methods

HSCs undergoing transdifferentiation were incubated with ethanol and expression of fibrogenic genes and epigenetic regulators was measured. Mechanisms responsible for recorded changes were investigated using ChIP-Seq and bioinformatics analysis. Ethanol induced changes were confirmed using HSCs isolated from a mouse alcohol mo

SUBMITTER: Page A 

PROVIDER: S-EPMC4629846 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature