Proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket.

ABSTRACT: Periodontal infections cause inflammatory destruction of the tooth supporting tissues. We recently developed a dynamic, in vitro periodontal organotypic tissue model in a perfusion bioreactor system, in co-culture with an 11-species subgingival biofilm, which may recapitulate early events during the establishment of periodontal infections. This study aimed to characterize the global proteome regulations in this host-biofilm interaction model. Semi-quantitative shotgun proteomics were applied for protein identification and quantification in the co-culture supernatants (human and bacterial) and the biofilm lysates (bacterial). A total of 896 and 3363 proteins were identified as secreted in the supernatant and expressed in the biofilm lysate, respectively. Enriched gene ontology analysis reve

SUBMITTER: Bao K 

PROVIDER: S-EPMC4630604 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature