Identification of novel tylosin analogues generated by a wblA disruption mutant of Streptomyces ansochromogenes.

ABSTRACT:

Background

Streptomyces, as the main source of antibiotics, has been intensively exploited for discovering new drug candidates to combat the evolving pathogens. Disruption of wblA, an actinobacteria-specific gene controlling major developmental transition, can cause the alteration of phenotype and morphology in many species of Streptomyces. One wblA homologue was found in Streptomyces ansochromogenes 7100 by using the Basic Local Alignment Search Tool. It is interesting to identify whether novel secondary metabolites could be produced by the wblA disruption mutant as evidenced in other Streptomyces.

Results

The wblA disruption mutant of S. ansochromogenes 7100 (ΔwblA) was constructed by homologous recombination. ΔwblA failed to produce spores and nikkomycin, the major produc

SUBMITTER: Lu C 

PROVIDER: S-EPMC4630966 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature