Evolutionary rescue by compensatory mutations is constrained by genomic and environmental backgrounds.

ABSTRACT: Since deleterious mutations may be rescued by secondary mutations during evolution, compensatory evolution could identify genetic solutions leading to therapeutic targets. Here, we tested this hypothesis and examined whether these solutions would be universal or would need to be adapted to one's genetic and environmental make-ups. We performed experimental evolutionary rescue in a yeast disease model for the Wiskott-Aldrich syndrome in two genetic backgrounds and carbon sources. We found that multiple aspects of the evolutionary rescue outcome depend on the genotype, the environment, or a combination thereof. Specifically, the compensatory mutation rate and type, the molecular rescue mechanism, the genetic target, and the associated fitness cost varied across contexts. The course of compen

SUBMITTER: Filteau M 

PROVIDER: S-EPMC4631203 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature