Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios.

ABSTRACT: The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n = 1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and ¹H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.

SUBMITTER: Manono J 

PROVIDER: S-EPMC4631223 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature