Competitive volumetric bar-chart chip with real-time internal control for point-of-care diagnostics.

ABSTRACT: Point-of-care (POC) testing has become widely used in clinical analysis because of its speed and portability; however, POC tools, such as lateral flow assays, suffer from low specificity, unclear readouts, and susceptibility to environmental and user errors. Herein, we report an ELISA-based competitive volumetric bar-chart chip (CV-chip) that eliminates these limitations. The CV-chip displays the readout in the form of ink bar charts based on direct competition between gases generated by the sample and the internal control. By employing a "competition mode", this platform decreases the potential influence of background resulting from environmental factors and provides visually clear positive or negative results without the requirement of calibration. In addition, the on-chip comparison ena

SUBMITTER: Li Y 

PROVIDER: S-EPMC4631400 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature