Tumor-targeted and pH-controlled delivery of doxorubicin using gold nanorods for lung cancer therapy.

ABSTRACT:

Background

In lung cancer, the efficacy of conventional chemotherapy is limited due to poor drug accumulation in tumors and nonspecific cytotoxicity. Resolving these issues will increase therapeutic efficacy.

Methods

GNR-Dox-Tf-NPs (gold nanorod-doxorubicin-transferrin-nanoparticles) were prepared by different chemical approaches. The efficacy of these nanoparticles was carried out by cell viability in lung cancer and primary coronary artery smooth muscle cells. The receptor-mediated endocytosis studies were done with human transferrin and desferrioxamine preincubation. The GNR-Dox-Tf nanoparticles induced apoptosis, and DNA damage studies were done by Western blot, H2AX foci, and comet assay.

Results

We developed and tested a gold nanorod-based multifunctional nanopa

SUBMITTER: Amreddy N 

PROVIDER: S-EPMC4631428 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature