Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics.

ABSTRACT: Interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of scientific applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, our microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression with single hyphal co

SUBMITTER: Geng T 

PROVIDER: S-EPMC4632079 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature