Polystyrene microspheres enable 10-color compensation for immunophenotyping of primary human leukocytes.

ABSTRACT: Compensation is a critical process for the unbiased analysis of flow cytometry data. Numerous compensation strategies exist, including the use of bead-based products. The purpose of this study was to determine whether beads, specifically polystyrene microspheres (PSMS) compare to the use of primary leukocytes for single color based compensation when conducting polychromatic flow cytometry. To do so, we stained individual tubes of both PSMS and leukocytes with panel specific antibodies conjugated to fluorochromes corresponding to fluorescent channels FL1-FL10. We compared the matrix generated by PSMS to that generated using peripheral blood mononuclear cells (PBMC). Ideal for compensation is a sample with both a discrete negative population and a bright positive population. We demonstrate t

SUBMITTER: Byrd T 

PROVIDER: S-EPMC4632493 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature